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Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
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JoVE Journal Neurociência
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

5,894 Views

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07:35 min

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August 06, 2019

DOI:

10.3791/59797-v

DOI:
10.3791/59797-v

•

07:35 min
August 06, 2019

•

4 Views
,
1Laboratory of RNA Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, 2Department of Genetics and Microbiology, Faculty of Science,Charles University

Summary

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Biogenesis of spliceosomal snRNAs is a complex process involving various cellular compartments. Here, we employed microinjection of fluorescently labelled snRNAs in order to monitor their transport inside the cell.

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Cite this Article

Roithová, A., Staněk, D. Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection. J. Vis. Exp. (150), e59797, doi:10.3791/59797 (2019).

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