Journal
/
Bioquímica
/
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

DOI:
10.3791/59773-v

•

00:07 min

•

August 30, 2019

•

, , ,
1Nebraska Center for Virology, 2School of Biological Sciences,University of Nebraska-Lincoln, 3Department of Food Science and Technology,University of Nebraska-Lincoln

Capítulos

  • 00:04Título
  • 00:41Bone Marrow, Liver, Thymus-Humanized (hu-BLT) Mouse Generation
  • 03:43Human Immune Cell Level Evaluation and Fresh Fecal Sample Collection
  • 05:20Fecal Transplant
  • 06:05Results: Representative Double Humanized BLT-Mouse Gut Microbiome Analyses
  • 07:02Conclusion

Summary

Tadução automática

Tadução automática

We describe a novel method for generating double humanized BLT-mice that feature a functional human immune system and a stable engrafted human-like gut microbiome. This protocol can be followed without the need for germ-free mice or gnotobiotic facilities.

Tags

Double Humanized MiceHuman Gut MicrobiomeHuman Immune SystemBiomedical ResearchHematopoietic Stem CellsTransplantationSurgery Procedure

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Analysis and Specification of Starch Granule Size Distributions

Read Article