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使用含有微型基因的Alu元素来分析圆形RNA

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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使用含有微型基因的Alu元素来分析圆形RNA

DOI:

10.3791/59760-v

•

13:10 min

•

March 10, 2020

•

Justin R. Welden, Anna Pawluchin, Jacob van Doorn, Stefan Stamm

¹Institute for Molecular and Cellular Biochemistry,University of Kentucky

Capítulos

00:05Introduction
01:09Design of the Constructs
02:14Select the Sequence to be Cloned in an Expression Vector
03:35Design Primers for Cloning
04:59Cloning of Constructs
07:05Transfection of the Reporter Genes into Eukaryotic Cells
10:21Results: Analysis of Circular RNAs
11:09Conclusion

Summary

Tadução automática

我们克隆和分析生成圆形RNA的记者基因。这些报告基因大于构造，用于分析线性拼接并包含Alu元素。为了研究圆形RNA，构造被转染到细胞中，在去除线性RNA后使用RT-PCR分析产生的RNA。

Tags

Keywords: Circular RNA Alternative Splicing Genomic Expression System Alu Element PCR Optimization UCSC Genome Browser Repetitive Elements Primer Design Exon-intron Borders Reverse Complement

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