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Use of Alu Element Containing Minigenes to Analyze Circular RNAs
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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13:10 min

March 10, 2020

DOI:

13:10 min
March 10, 2020

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Summary

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We clone and analyze reporter genes generating circular RNAs. These reporter genes are larger than constructs to analyze linear splicing and contain Alu elements. To investigate the circular RNAs, the constructs are transfected into cells and resulting RNA is analyzed using RT-PCR after removal of linear RNA.

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