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在Vivo前向遗传屏幕识别新的神经保护基因在果蝇黑色素
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JoVE Journal Genética
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
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在Vivo前向遗传屏幕识别新的神经保护基因在果蝇黑色素

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10:00 min

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July 11, 2019

DOI:

10.3791/59720-v

DOI:
10.3791/59720-v

•

10:00 min
July 11, 2019

•

8 Views
, , , , , , , , ,
1Department of Biological Sciences,University of Alabama, 2Department of Genetics,University of Wisconsin-Madison

Summary

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我们提出了一个协议,使用前向遗传方法筛选在果蝇黑色素细胞中表现出神经退化的突变体。它结合了爬升测定、组织分析、基因映射和DNA测序,最终鉴定出与神经保护过程相关的新基因。

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Cite this Article

Gevedon, O., Bolus, H., Lye, S. H., Schmitz, K., Fuentes-González, J., Hatchell, K., Bley, L., Pienaar, J., Loewen, C., Chtarbanova, S. In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster. J. Vis. Exp. (149), e59720, doi:10.3791/59720 (2019).

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