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近红外荧光和高分辨率扫描测量啮齿类动物大脑中蛋白质的表达

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

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近红外荧光和高分辨率扫描测量啮齿类动物大脑中蛋白质的表达

DOI:

10.3791/59685-v

•

06:04 min

•

May 23, 2019

•

Brianna Kimmelmann-Shultz, Negin Mohmammadmirzaei, Jeffrey Caplan, Dayan Knox

¹Department of Psychological and Brain Sciences,University of Delaware, ²Plant and Soil Sciences, Delaware Biotechnology Institute,University of Delaware

Capítulos

00:04Título
00:45Single Immunohistochemical Reaction
02:45Imaging and Protein Expression Analysis
04:14Results: Detection and Measurement of Protein Expression in the Rodent Brain
05:30Conclusion

Summary

Tadução automática

在这里, 我们提出了一个协议, 使用近红外染料结合免疫组织化学和高分辨率扫描来检测大脑区域的蛋白质。

Tags

Keywords: Near-infrared Fluorescence High-resolution Scanning Protein Expression Rodent Brain Immunohistochemistry Molecular Signaling Pathways AMPA/NMDA Ratio Cryostat Paraformaldehyde TBS Primary Antibody Secondary Antibody Near-infrared Scanning

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