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Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain
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Neurociência
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JoVE Journal Neurociência
Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

DOI:
10.3791/59685-v

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06:04 min

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May 23, 2019

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, , ,
1Department of Psychological and Brain Sciences,University of Delaware, 2Plant and Soil Sciences, Delaware Biotechnology Institute,University of Delaware

Capítulos

  • 00:04Título
  • 00:45Single Immunohistochemical Reaction
  • 02:45Imaging and Protein Expression Analysis
  • 04:14Results: Detection and Measurement of Protein Expression in the Rodent Brain
  • 05:30Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a protocol that uses near-infrared dyes in conjunction with immunohistochemistry and high-resolution scanning to assay proteins in brain regions.

Tags

Keywords: Near-infrared FluorescenceHigh-resolution ScanningProtein ExpressionRodent BrainImmunohistochemistryMolecular Signaling PathwaysAMPA/NMDA RatioCryostatParaformaldehydeTBSPrimary AntibodySecondary AntibodyNear-infrared Scanning

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