Journal
/
/
用于长期无细胞基因表达传导的多层微流体平台
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
DOI:

11:23 min

October 06, 2019

, , , , , ,

Capítulos

  • 00:04Título
  • 01:00Off-Chip Cooling Setup
  • 02:35Preparing the Setup for an Experiment
  • 04:20Connecting Uncooled Reagents to the Device
  • 05:45Connecting Cooled Fluids to the Microfluidic Device
  • 06:49Loading the Microfluidic Device
  • 08:09Calibrating the Microfluidic Device
  • 08:56Results: IVTT Experiment Expressing the deGFP Protein
  • 10:40Conclusion

Summary

Tadução automática

介绍了基于PDMS的多层微流体装置的制造过程,该装置允许长时间进行体外转录和翻译(IVTT)反应。此外,还全面概述了自动化和维护这些反应所需的硬件和软件,

Vídeos Relacionados

Read Article