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A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
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A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
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October 06, 2019
DOI:
1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group,Eindhoven University of Technology, 2Institute for Molecules and Materials,Radboud University, 3Institute of Bioengineering,School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)
Summary
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The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.
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van der Linden, A. J., Yelleswarapu, M., Pieters, P. A., Swank, Z., Huck, W. T. S., Maerkl, S. J., de Greef, T. F. A. A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression. J. Vis. Exp. (152), e59655, doi:10.3791/59655 (2019).
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