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Opsporen en karakteriseren van zelf-assemblage van eiwitten in vivo door flow Cytometrie

JoVE Journal
Biologia

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JoVE Journal Biologia

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

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Opsporen en karakteriseren van zelf-assemblage van eiwitten in vivo door flow Cytometrie

10,538 Views

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05:58 min

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July 17, 2019

DOI:

10.3791/59577-v

DOI:

10.3791/59577-v

•

05:58 min

July 17, 2019

•

5 Views

Shriram Venkatesan*¹, Tejbir S. Kandola*¹, Alejandro Rodríguez-Gama, Andrew Box, Randal Halfmann²

¹Stowers Institute for Medical Research, ²Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Tags

Protein Self-assembly Flow Cytometry DAmFRET FRET Acceptor Signal Yeast Culture Induction Medium Photo Conversion Imaging Flow Cytometer Fluorescence Positive Gate

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