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巨型囊泡内单细胞层的细菌细胞培养

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

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巨型囊泡内单细胞层的细菌细胞培养

DOI:

10.3791/59555-v

•

07:33 min

•

April 30, 2019

•

Masamune Morita, Yuri Ota², Kaoru Katoh, Naohiro Noda²

¹Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology (AIST), ²Department of Life Science and Medical Bioscience,Waseda University

Capítulos

00:04Título
00:31Lipid-containing Oil Solution and Small Vesicle (SV) Preparation
01:54Bacterial Cell Pre-culture
02:45Giant Vesicle (GV) and Water-in-Oil (W/O) Bacterial Cell-containing Droplet Formation
04:11Handmade Chamber and Supported Bilayer Membrane Preparation
04:54GV Immobilization and Microscopic Bacterial Growth Observation
06:04Results: Representative GV Containing Bacteria and Bacterial Growth Evaluation
06:44Conclusion

Summary

Tadução automática

我们演示了巨型囊泡 (GV) 内的细菌的单细胞培养。含有细菌细胞的GV通过滴转移方法制备,固定在玻璃基板上的支撑膜上,直接观察细菌生长。这种方法也可以适应其他细胞。

Tags

Keywords: Bacterial Cell Culture Single-cell Level Giant Vesicles POPC Biotin-PEG-DSPE Mineral Oil Sonication Extrusion E. Coli LB Medium Optical Density Sucrose Solution Outer Aqueous Solution Lipid-containing Oil Solution Water-oil Droplet Formation

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