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カルボキシフルオレスチンスクシニミジルエステルを用いたヒト抗原特異的CD4+T細胞の増殖の定量化
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
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カルボキシフルオレスチンスクシニミジルエステルを用いたヒト抗原特異的CD4+T細胞の増殖の定量化

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07:00 min

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June 04, 2019

DOI:

10.3791/59545-v

DOI:
10.3791/59545-v

•

07:00 min
June 04, 2019

•

8 Views
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1Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, 2Department of Medicine,University of Melbourne, St. Vincent’s Hospital

Tags

CD4+ T CellsProliferationCFSEAntigen-specificQuantificationAutoimmune ConditionsType 1 DiabetesPBMCDensity Gradient CentrifugationCell CultureFlow Cytometry

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Di Carluccio, A. R., Tresoldi, E., So, M., Mannering, S. I. Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester. J. Vis. Exp. (148), e59545, doi:10.3791/59545 (2019).

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