Quantification of Proliferating Human Antigen-specific CD4<sup>+</sup> T Cells using Carboxyfluorescein Succinimidyl Ester

Anthony R. Di Carluccio; Eleonora Tresoldi; Michelle So; Stuart I. Mannering

doi:10.3791/59545

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免疫学と感染

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Quantification of Proliferating Human Antigen-specific CD4⁺ T Cells using Carboxyfluorescein Succinimidyl Ester

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JoVE Journal 免疫学と感染

Quantification of Proliferating Human Antigen-specific CD4⁺ T Cells using Carboxyfluorescein Succinimidyl Ester

8,140 Views

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07:00 min

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June 04, 2019

DOI:

10.3791/59545-v

DOI:

10.3791/59545-v

•

07:00 min

June 04, 2019

•

13 Views

Anthony R. Di Carluccio¹, Eleonora Tresoldi¹, Michelle So¹, Stuart I. Mannering^1,2

¹Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, ²医学部,University of Melbourne, St. Vincent’s Hospital

概要

Presented here is a protocol for measuring proliferating CD4⁺ T cells in response to antigenic proteins or peptides using dye dilution. This assay is particularly sensitive to rare antigen-specific T cells and can be modified to facilitate cloning of antigen-specific cells.

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Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

概要

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Quantification of Proliferating Human Antigen-specific CD4⁺ T Cells using Carboxyfluorescein Succinimidyl Ester