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Isotropisk lys-Sheet mikroskopi og automatiseret celle Lineage analyser til kataloget Caenorhabditis elegans Embryogenesis med Subcellular opløsning
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
DOI:

08:16 min

June 06, 2019

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Capítulos

  • 00:04Título
  • 00:34C. elegans Embryo Sample Mounting Preparation
  • 01:48Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
  • 03:12Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
  • 06:06Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
  • 07:43Conclusion

Summary

Tadução automática

Her præsenterer vi en kombinatorisk tilgang ved hjælp af høj opløsning mikroskopi, beregningsmæssige værktøjer, og enkelt celle mærkning i levende C. elegans embryoner til at forstå enkelt celle dynamik under neurodevelopment.

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