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Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
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June 06, 2019
DOI:
1Department of Neuroscience and Department of Cell Biology,Yale University School of Medicine, 2Developmental Biology Program,Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling,University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas,Universidad de Puerto Rico
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Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment.
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Duncan, L. H., Moyle, M. W., Shao, L., Sengupta, T., Ikegami, R., Kumar, A., Guo, M., Christensen, R., Santella, A., Bao, Z., Shroff, H., Mohler, W., Colón-Ramos, D. A. Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution. J. Vis. Exp. (148), e59533, doi:10.3791/59533 (2019).
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