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JoVE Journal Neuroscience
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
 

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Article DOI: 10.3791/59526-v • 10:41 min • June 7th, 2019
June 7th, 2019 •
*1, *1, 1, 1
1Vollum Institute, Oregon Health & Science University
* These authors contributed equally

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A procedure is presented to visualize protein kinase A activities in head-fixed, behaving mice. An improved A-kinase activity reporter, tAKARα, is expressed in cortical neurons and made accessible for imaging through a cranial window. Two-photon fluorescence lifetime imaging microscopy is used to visualize PKA activities in vivo during enforced locomotion.

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Keywords: Protein Kinase A PKA TAKARα Two-photon Fluorescence Lifetime Imaging Microscopy 2pFLIM Neuromodulation CAMP FRET Head-fixed Behavior In Vivo Imaging Motor Cortex
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