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原子吸纳光谱测量哺乳动物细胞细胞内锌池
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JoVE Journal Bioquímica
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
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原子吸纳光谱测量哺乳动物细胞细胞内锌池

DOI:
10.3791/59519-v

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13:04 min

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May 16, 2019

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, , , , ,
1Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Department of Chemistry,Skidmore College, 3Candiac MR Center,Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Capítulos

  • 00:04Título
  • 02:51Mammalian Cell Culture Sample Preparation for AAS (Atomic Absorption Spectrometry): Whole Cell and Subcellular Fractionation
  • 05:59Zn Content Analysis of Whole Cell and Subcellular Fractions of Mammalian Cell Cultures by AAS
  • 09:28Results: Purity of Subcellular Fractions and Zinc Levels in Different Mammalian Cultured Cells
  • 11:11Conclusion

Summary

Tadução automática

Tadução automática

培养的原生细胞系或已建立的细胞系通常用于在使用动物模型之前解决基本的生物和机械问题, 作为初步方法。该方案介绍了如何利用原子吸收光谱制备用于锌 (Zn) 和其他微量元素研究的全细胞提取物和亚细胞组分。

Tags

Atomic Absorbance SpectroscopyIntracellular ZincMammalian CellsTransition MetalsMetal HomeostasisGraphite Furnace Atomic Absorption Spectroscopy (GF-AAS)Trace Element AnalysisMetal MisbalancesPathological ConditionsMethod DevelopmentSample PreparationLow Sample VolumeMethod Validation

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