Journal
/
Genética
/
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
JoVE Journal
Genética
This content is Free Access.
JoVE Journal Genética
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

DOI:
10.3791/59512-v

•

08:31 min

•

June 20, 2019

•

, , , , , , ,
1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine,University of Michigan Medical Center, 2Celetrix LLC

Capítulos

  • 00:04Título
  • 01:08Preparation of Cells
  • 02:51Tube Electroporation of Cas9 RNP and ssODNs
  • 04:24Analysis of Gene Editing Events
  • 05:48Results: Results of Tube Electroporation
  • 07:59Conclusion

Summary

Tadução automática

Tadução automática

Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.

Tags

Keywords: CRISPR/Cas9Gene EditingTube ElectroporationCell TransfectionIPSCsRabbit FibroblastsGRNADonor OligosCell CultureElectroporation Buffer

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/59512?article_type=v

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
check_url/pt/59512?article_type=v

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
check_url/pt/59512?article_type=v

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
check_url/pt/59512?article_type=v

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
check_url/pt/59512?article_type=v

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
check_url/pt/59512?article_type=v

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
check_url/pt/59512?article_type=v

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
check_url/pt/59512?article_type=v

CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
check_url/pt/59512?article_type=v

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
check_url/pt/59512?article_type=v

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Read Article