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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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Genetik
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JoVE Journal Genetik
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

DOI:
10.3791/59512-v

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08:31 min

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June 20, 2019

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, , , , , , ,
1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine,University of Michigan Medical Center, 2Celetrix LLC

Kapitel

  • 00:04Titel
  • 01:08Preparation of Cells
  • 02:51Tube Electroporation of Cas9 RNP and ssODNs
  • 04:24Analysis of Gene Editing Events
  • 05:48Results: Results of Tube Electroporation
  • 07:59Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.

Tags

CRISPR/Cas9Gene EditingTube ElectroporationCell TransfectionIPSCsRabbit FibroblastsGRNADonor OligosCell CultureElectroporation Buffer

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