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An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
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1Department of Endocrinology & Metabolism Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 2Berlin Institute of Health (BIH), 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 4DZHK (German Centre for Cardiovascular Research), 5Institute of Medical Immunology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 6Julius Wolff Institute (JWI) and Center for Musculoskeletal Surgery, Charité – Universitätsmedizin Berlin, Berlin, Germany, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin
Capítulos
- 00:04Título
- 00:58Adipose Tissue Digestion
- 03:37Liver Tissue Digestion
- 05:56Surface Staining and Flow Cytometric Analysis
- 07:53Results: Representative Leukocyte Population Analysis in a Nonalcoholic Steatohepatitis Mouse Model
- 09:46Conclusion
A simple and reliable diet-induced rodent animal model for nonalcoholic steatohepatitis (NASH) is described, achieved through non-SPF housing of the animals and administration of a specific high-fat diet. We describe identification of hepatic and adipose immune cell subsets to recapitulate human immunological conditions by exposing mice to environmental germs.
