/
/
An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
6,839 Views
•
10:17 min
•
April 26, 2019
DOI:
1Department of Endocrinology & Metabolism Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 2Berlin Institute of Health (BIH), 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 4DZHK (German Centre for Cardiovascular Research), 5Institute of Medical Immunology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin, 6Julius Wolff Institute (JWI) and Center for Musculoskeletal Surgery, Charité – Universitätsmedizin Berlin, Berlin, Germany, corporate member of Freie Universität Berlin,Humboldt-Universität zu Berlin
Summary
Automatically generated
A simple and reliable diet-induced rodent animal model for nonalcoholic steatohepatitis (NASH) is described, achieved through non-SPF housing of the animals and administration of a specific high-fat diet. We describe identification of hepatic and adipose immune cell subsets to recapitulate human immunological conditions by exposing mice to environmental germs.
Read Article
Cite this Article
Sbierski-Kind, J., Schmidt-Bleek, K., Streitz, M., Kath, J., Spranger, J., Volk, H. An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes. J. Vis. Exp. (146), e59470, doi:10.3791/59470 (2019).
Copy