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Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

5,551 Views

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06:18 min

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April 26, 2019

DOI:

10.3791/59453-v

DOI:
10.3791/59453-v

•

06:18 min
April 26, 2019

•

3 Views
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1Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases,Tsinghua University School of Medicine

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MycobacteriumMistranslation RatesGain-of-function ReporterNluc/GFP AssayAntibiotic ToleranceProtein Synthesis Errors7H9 MediumOD 600ATCKsgPassive Lysis BufferFirefly SubstrateRenilla SubstrateLuminometer

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Chen, Y., Pan, M., Chen, Y., Javid, B. Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems. J. Vis. Exp. (146), e59453, doi:10.3791/59453 (2019).

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