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DREADD制御神経活動の慢性操作に対する非侵襲的戦略
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Neurociência
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JoVE Journal Neurociência
Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity
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DREADD制御神経活動の慢性操作に対する非侵襲的戦略

12,720 Views

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00:08 min

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August 25, 2019

DOI:

10.3791/59439-v

DOI:
10.3791/59439-v

•

00:08 min
August 25, 2019

•

23 Views
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1Section on Light and Circadian Rhythms (SLCR),National Institute of Mental Health, 2Howard Hughes Medical Institute, Department of Neuroscience,Scripps Research Institute

Summary

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ここでは、マウスの化学遺伝学を用いて神経活動を慢性的に制御する2つの非侵襲的方法について説明する。点眼剤は、クロザピン-N-酸化物(CNO)を毎日提供するために使用されました。また、飲料水中のCNOの長期投与のための2つの方法についても述べた。慢性神経制御のためのこれらの戦略は、動物のストレスを軽減する最小限の介入を必要とします。

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Cite this Article

Zhan, J., Komal, R., Keenan, W. T., Hattar, S., Fernandez, D. C. Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity. J. Vis. Exp. (150), e59439, doi:10.3791/59439 (2019).

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