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Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity
JoVE Journal
神経科学
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JoVE Journal 神経科学
Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity

Non-invasive Strategies for Chronic Manipulation of DREADD-controlled Neuronal Activity

DOI:

00:08 min

August 25, 2019

, , , ,

  • 00:04標題
  • 00:53Hippocampal Adeno-associated Virus Injection
  • 02:10Repetitive Clozapine-N-Oxide (CNO) Delivery
  • 03:20Chronic CNO Treatment
  • 04:07Restricted CNO Treatment
  • 05:01Data Analysis
  • 06:15Results: Representative Effects of Repetitive and Chronic CNO Delivery
  • 07:40Conclusion

概要

自動翻訳

Here we describe two non-invasive methods to chronically control neuronal activity using chemogenetics in mice. Eye-drops were used to deliver clozapine-N-oxide (CNO) daily. We also describe two methods for prolonged administration of CNO in drinking water. These strategies for chronic neuronal control require minimal intervention reducing animals’ stress.

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