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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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JoVE Journal Genética
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

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10:10 min

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March 31, 2019

DOI:

10.3791/59382-v

DOI:
10.3791/59382-v

•

10:10 min
March 31, 2019

•

1 Views
, , , , , ,
1Department of Pediatrics,Pennsylvania State University College of Medicine, 2Department of Physiological Sciences,University of Florida, 3Department of Anatomy and Cell Biology,University of Florida

Summary

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A CRISPR/sgRNA library has been applied to interrogating protein-coding genes.However, the feasibility of a sgRNA library to uncover the function of a CTCF boundary in gene regulation remains unexplored. Here, we describe a HOX loci specific sgRNA library to elucidate the function of CTCF boundaries in HOX loci.

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Cite this Article

Luo, H., Sobh, A., Vulpe, C. D., Brewer, E., Dovat, S., Qiu, Y., Huang, S. HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries. J. Vis. Exp. (145), e59382, doi:10.3791/59382 (2019).

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