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Imunologia e Infecção
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Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

DOI:
10.3791/59357-v

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07:20 min

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April 09, 2019

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, , ,
1Department of Internal Medicine,University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology,Johns Hopkins University School of Medicine

Capítulos

  • 00:04Título
  • 01:22Isolation and Dissociation of Enteroids/Colonoids from 3D Culture
  • 02:39Plating the Enteroid/Colonoid Suspension
  • 04:10Fixation to Preserve and Immunostain Mucus
  • 05:16Results: Establishment and Evaluation of Human Enteroid/Colonoid Monolayers
  • 06:51Conclusion

Summary

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Tadução automática

Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

Tags

Colonoid MonolayersIntestinal EpitheliumHost-pathogen InteractionMucus BiologyMicrobiomeInflammatory Bowel Disease3D Organoid CultureEx Vivo StudiesHarvesting SolutionCell SuspensionDMEM/F12 MediumCell PlatingExpansion Medium

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