/
/
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
This content is Free Access.
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
16,302 Views
•
10:18 min
•
May 28, 2019
DOI:
Summary
Automatically generated
The CRISPR/Cas12a system in combination with a single crRNA array enables efficient multiplex editing of the S. cerevisiae genome at multiple loci simultaneously. This is demonstrated by constructing carotenoid producing yeast strains which are subsequently used to create yeast pixel art.
Read Article
Cite this Article
Ciurkot, K., Vonk, B., Gorochowski, T. E., Roubos, J. A., Verwaal, R. CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art. J. Vis. Exp. (147), e59350, doi:10.3791/59350 (2019).
Copy