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线虫中耳炎群链球菌引起氧化应激的研究

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

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线虫中耳炎群链球菌引起氧化应激的研究

DOI:

10.3791/59301-v

•

09:36 min

•

March 23, 2019

•

Ali Naji, Ali Al Hatem, Ransome van der Hoeven

¹Department of Diagnostic and Biomedical Sciences, School of Dentistry,University of Texas Health Science Center

Capítulos

00:04Título
00:29Preparation of THY (Todd-Hewitt Yeast Extract) Agar Plates and Mitis Group Streptococci
02:12Survival Assays
04:04Preparation of Agarose Pads for Microscopy
04:52Observation of SKN-1 Localization in Response to Streptococcus Infection
07:36Results: Killing of the Worms
09:02Conclusion

Summary

Tadução automática

线虫是解剖寄主-病原体相互作用的良好模型。这里描述的是一个协议, 感染蠕虫与炎组链球菌成员, 并确定激活的氧化应激反应对 h2o2 产生的这一组生物。

Tags

Keywords: Oxidative Stress Mitis Group Streptococci Caenorhabditis Elegans Hydrogen Peroxide Pathogenicity Stress Response Reactive Oxygen Species Todd Hewitt Agar THY Agar Catalase E. Coli OP-50 NGM Plates Microaerophilic Environment

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