/
/
Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans
Capítulos
- 00:04Título
- 00:29Preparation of THY (Todd-Hewitt Yeast Extract) Agar Plates and Mitis Group Streptococci
- 02:12Survival Assays
- 04:04Preparation of Agarose Pads for Microscopy
- 04:52Observation of SKN-1 Localization in Response to Streptococcus Infection
- 07:36Results: Killing of the Worms
- 09:02Conclusion
The nematode Caenorhabditis elegans is an excellent model to dissect host-pathogen interactions. Described here is a protocol to infect the worm with members of the mitis group streptococci and determine activation of the oxidative stress response against H2O2 produced by this group of organisms.
