/
/
Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
12,839 Views
•
09:54 min
•
April 18, 2019
DOI:
1CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine,The Catholic University of Korea, 2Department of Biomedicine & Health Science, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 3Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea
Summary
Automatically generated
We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.
Read Article
Cite this Article
Kim, Y., Ju, J. H. Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells. J. Vis. Exp. (146), e59297, doi:10.3791/59297 (2019).
Copy