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Mikroinjektion-baseret System for i Vivo Implantation af embryonale Cardiomyocytes i aviær Embryo
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Capítulos
- 00:04Título
- 00:49Micro-Injection Pipette Preparation
- 01:53Host Embryo Preparation
- 03:34Donor Tissue Isolation
- 04:44Donor Tissue Digestion
- 05:50In Vivo Injection
- 07:37Results: Representative Donor Myocyte-Implanted Embryonic Recipient Heart Imaging
- 08:14Conclusion
I denne metode er embryonale hjerte væv bortopereret microdissected, adskilles, fluorescently mærket og implanteret i vært embryonale væv. Dette giver en platform for at studere de enkelte eller væv niveau udviklingsmæssige organisation under ektopisk hæmodynamiske forhold, og/eller ændret paracrine/juxtacrine miljøer.
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Keyword Extraction:
MicroinjectionIn Vivo ImplantationEmbryonic CardiomyocytesAvian EmbryoBiomechanicsParacrine EnvironmentsJuxtacrine InteractionsCellular PhenotypeExperimental EmbryologyGraphing ExperimentsInjection PressureInjection EquipmentGlass CapillariesSiliconizing AgentMicroinjection PipetteFertile Chicken EggsAlbumenTransparent TapeHamburger And HamiltonIndia InkHBSS
