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Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
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February 17, 2019
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In this method, embryonic cardiac tissues are surgically microdissected, dissociated, fluorescently labeled, and implanted into host embryonic tissues. This provides a platform for studying the individual or tissue level developmental organization under ectopic hemodynamic conditions, and/or altered paracrine/juxtacrine environments.
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Keyword Extraction:MicroinjectionIn Vivo ImplantationEmbryonic CardiomyocytesAvian EmbryoBiomechanicsParacrine EnvironmentsJuxtacrine InteractionsCellular PhenotypeExperimental EmbryologyGraphing ExperimentsInjection PressureInjection EquipmentGlass CapillariesSiliconizing AgentMicroinjection PipetteFertile Chicken EggsAlbumenTransparent TapeHamburger And HamiltonIndia InkHBSS
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Henley, T., Thomas, K., Bressan, M. Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo. J. Vis. Exp. (144), e59267, doi:10.3791/59267 (2019).
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