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基于微注射的免疫胚胎心肌细胞体内植入系统

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo

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基于微注射的免疫胚胎心肌细胞体内植入系统

DOI:

10.3791/59267-v

•

08:52 min

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February 17, 2019

•

Trevor Henley, Kandace Thomas, Michael Bressan

¹Department of Cell Biology and Physiology, McAllister Heart Institute,University of North Carolina at Chapel Hill

Capítulos

00:04Título
00:49Micro-Injection Pipette Preparation
01:53Host Embryo Preparation
03:34Donor Tissue Isolation
04:44Donor Tissue Digestion
05:50In Vivo Injection
07:37Results: Representative Donor Myocyte-Implanted Embryonic Recipient Heart Imaging
08:14Conclusion

Summary

Tadução automática

在这种方法中, 胚胎心脏组织被手术显微解剖, 离解, 荧光标记, 并植入宿主胚胎组织。这为研究异位血流动力学条件下的个体或组织水平发育组织以及/或改变后的副血流/朱克沙林环境提供了一个平台。

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