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Biologia do Desenvolvimento

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达尼奥胚胎发生过程中的上眼硫可视化

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis

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达尼奥胚胎发生过程中的上眼硫可视化

DOI:

10.3791/59259-v

•

08:03 min

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March 27, 2019

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Kevin H. Yoon, Sonya A. Widen², Melissa M. Wilson, Jennifer C. Hocking^3,4,5, Andrew J. Waskiewicz^2,6

¹Department of Biological Sciences,University of Alberta, ²Women & Children’s Health Research Institute,University of Alberta, ³Division of Anatomy, Department of Surgery,University of Alberta, ⁴Department of Cell Biology,University of Alberta, ⁵Department of Medical Genetics,University of Alberta, ⁶Neuroscience and Mental Health Research Institute,University of Alberta

Capítulos

00:04Título
00:28Superior Ocular Sulcus (SOS) Visualization
03:15Whole-Mount Immunofluorescent Laminin Staining
06:04Results: Representative Zebrafish SOS Imaging
07:31Conclusion

Summary

Tadução automática

在这里, 我们提出了一系列标准化的协议, 以观察上眼沟, 最近确定的, 进化保存的结构在脊椎动物的眼睛。利用斑马鱼幼虫, 我们展示了必要的技术, 以确定有助于形成和关闭的上眼沟的因素。

Tags

Keywords: Zebrafish Embryogenesis Superior Ocular Sulcus SOS Visualization Dissecting Microscope Compound Microscope Confocal Microscope 1-Phenyl-2-thiourea Agarose E3 Medium Embryo Positioning MRNA Injection Enhanced GFP Fluorescent Microscopy

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