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Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells
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Neurociência
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JoVE Journal Neurociência
Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells

DOI:
10.3791/59237-v

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08:52 min

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July 24, 2019

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, , , ,
1Department of Pharmacology,University of North Carolina Chapel Hill, 2Neuroscience Center,University of North Carolina Chapel Hill, 3Neuroscience Curriculum,University of North Carolina Chapel Hill

Capítulos

  • 00:04Título
  • 00:40Antigen Retrieval
  • 02:18Thymidine Analog Staining
  • 03:26Mounted Tissue Section Protocol
  • 05:02Image Analysis
  • 07:02Results: Analysis of Proliferating Neural Stem Cells and Progeny
  • 08:12Conclusion

Summary

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The goal of this protocol is to describe an approach for analyzing behavior of adult neural stem/progenitor cells in response to chemogenetic manipulation of a specific local neural circuit.

Tags

Adult NeurogenesisNeural CircuitsNeural Stem CellsProliferationBrain RegionsCircuit-specificThymidine AnalogEdU StainingImmunohistochemistry

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