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remtoe 控制系统在内源基因表达调控中的应用
JoVE Journal
Genética
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JoVE Journal Genética
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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remtoe 控制系统在内源基因表达调控中的应用

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08:54 min

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March 29, 2019

DOI:

10.3791/59235-v

DOI:
10.3791/59235-v

•

08:54 min
March 29, 2019

•

1 Views
, , , , ,
1Center for Epigenetics,Van Andel Research Institute, 2Amgen

Summary

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该协议概述了生成模型系统所需的步骤, 在该模型系统中, 相关内源基因的转录可以在活体动物或细胞中使用增强的 lac 抑制和/或 tet激活剂系统有条件地控制。

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Cite this Article

Vander Schaaf, N. A., Oghamian, S., Park, J., Kang, L., Laird, P. W., Lee, K. In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression. J. Vis. Exp. (145), e59235, doi:10.3791/59235 (2019).

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