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En menneskelige blod-hjerne Interface Model til at studere barriere overfarter af patogener eller lægemidler og deres interaktioner med hjernen
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Neurociência
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JoVE Journal Neurociência
A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

En menneskelige blod-hjerne Interface Model til at studere barriere overfarter af patogener eller lægemidler og deres interaktioner med hjernen

8,350 Views

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07:52 min

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April 09, 2019

DOI:

10.3791/59220-v

DOI:
10.3791/59220-v

•

07:52 min
April 09, 2019

•

4 Views
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1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS

Tags

Blood-brain InterfaceBlood-brain BarrierBBBPathogenMedicinaNeurosystemMini-brainEndothelial CellsLucifer YellowPermeabilityTransport BufferCHME Clone Five CellsNeuronsAstrocytesPolyester MembraneCell CultureCell SeedingCell CountingCell Trypsinization

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Cite this Article

da Costa, A., Prehaud, C., Bakoa, F., Afonso, P., Ceccaldi, P., Lafaye, P., Lafon, M. A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain. J. Vis. Exp. (146), e59220, doi:10.3791/59220 (2019).

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