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A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain
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Neurociencias
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JoVE Revista Neurociencias
A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

8,391 Views

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07:52 min

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April 09, 2019

DOI:

10.3791/59220-v

DOI:
10.3791/59220-v

•

07:52 min
April 09, 2019

•

6 Views
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1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS

Summary

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Here we present a protocol describing the setting of an in cellulo BBB (Blood brain barrier)-Minibrain polyester porous membrane culture insert system in order to evaluate the transport of biomolecules or infectious agents across a human BBB and their physiological impact on the neighboring brain cells.

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Cite this Article

da Costa, A., Prehaud, C., Bakoa, F., Afonso, P., Ceccaldi, P., Lafaye, P., Lafon, M. A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain. J. Vis. Exp. (146), e59220, doi:10.3791/59220 (2019).

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