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直接注射慢病毒载体突出大鼠脊髓中的多种运动途径

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord

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直接注射慢病毒载体突出大鼠脊髓中的多种运动途径

8,281 Views

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07:57 min

•

March 15, 2019

DOI:

10.3791/59160-v

DOI:

10.3791/59160-v

•

07:57 min

March 15, 2019

•

5 Views

Kathleen M. Keefe¹, Ian P. Junker¹, Imran S. Sheikh¹, Thomas J. Campion¹, George M. Smith¹

¹Shriner’s Pediatric Research Center,Temple University

Summary

该协议演示了在大鼠脊髓组织中注射一种具有可追溯性的可转移性病毒载体。载体在突触处被占据, 并被输送到目标神经元的细胞体。该模型适用于重要脊柱通路的逆行追踪或基因治疗应用中的靶向细胞。

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