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高圧急速凍結、マイクロ波を用いたコントラスト強調、およびボリューム イメージングのために埋め込む最小限の樹脂による生体試料作製
JoVE Journal
Biologia
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JoVE Journal Biologia
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
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高圧急速凍結、マイクロ波を用いたコントラスト強調、およびボリューム イメージングのために埋め込む最小限の樹脂による生体試料作製

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07:33 min

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March 19, 2019

DOI:

10.3791/59156-v

DOI:
10.3791/59156-v

•

07:33 min
March 19, 2019

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3 Views
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1Electron Microscopy Core Unit,Max Planck Institute of Experimental Medicine, 2Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Tags

High-pressure FreezingMicrowave-assisted Contrast EnhancementMinimal Resin EmbeddingVolume ImagingFocused Ion Beam Scanning Electron MicroscopeUltrastructure PreservationFreeze SubstitutionOsmium TetroxideUranyl AcetateSample PreparationNervus Tibialis

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Steyer, A. M., Ruhwedel, T., Möbius, W. Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging. J. Vis. Exp. (145), e59156, doi:10.3791/59156 (2019).

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