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Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
JoVE 杂志
生物学
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JoVE 杂志 生物学
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

9,807 Views

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07:33 min

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March 19, 2019

DOI:

10.3791/59156-v

DOI:
10.3791/59156-v

•

07:33 min
March 19, 2019

•

3 Views
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1Electron Microscopy Core Unit,Max Planck Institute of Experimental Medicine, 2Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

Tags

High-pressure FreezingMicrowave-assisted Contrast EnhancementMinimal Resin EmbeddingVolume ImagingFocused Ion Beam Scanning Electron MicroscopeUltrastructure PreservationFreeze SubstitutionOsmium TetroxideUranyl AcetateSample PreparationNervus Tibialis

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Steyer, A. M., Ruhwedel, T., Möbius, W. Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging. J. Vis. Exp. (145), e59156, doi:10.3791/59156 (2019).

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