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Biologia do Desenvolvimento
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Wholemount In Situ Hybridization for Astyanax Embryos
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Wholemount In Situ Hybridization for Astyanax Embryos

Wholemount In Situ Hybridization for Astyanax Embryos

DOI:
10.3791/59114-v

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09:56 min

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March 02, 2019

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, , ,
1Department of Biological Sciences,University of Cincinnati

Capítulos

  • 00:04Título
  • 00:40Day 1: Rehydration
  • 01:30Day 1: Digestion and Fixation
  • 02:42Day 1: Prehybridization and Day 2: Hybridization
  • 03:46Day 3: Solution Preparation and Probe Removal
  • 05:02Day 3: Blocking
  • 05:42Day 4: MABT Rinses
  • 06:34Day 5: Probe Visualization
  • 08:00Results: Analysis of Mounted Astyanax Embryos
  • 09:37Conclusion

Summary

Tadução automática

Tadução automática

This protocol enables visualization of gene expression in embryonic Astyanax cavefish. This approach has been developed with the goal of maximizing gene expression signal, while minimizing non-specific background staining.

Tags

Keywords: Wholemount In Situ HybridizationAstyanax EmbryosGene Expression PatternsProtocolPlatform ShakerProteinase KPBTPFAHybridization BufferWater Bath

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