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Karakterisering van Histone posttranslationele wijziging wijzigingen in gist neurodegeneratieve Proteinopathy modellen
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Genética
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JoVE Journal Genética
Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
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Karakterisering van Histone posttranslationele wijziging wijzigingen in gist neurodegeneratieve Proteinopathy modellen

7,319 Views

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08:33 min

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March 24, 2019

DOI:

10.3791/59104-v

DOI:
10.3791/59104-v

•

08:33 min
March 24, 2019

•

9 Views
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1Chemistry Department,Brooklyn College, 2Ph.D. Program in Biochemistry,Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology,Graduate Center of the City University of New York

Summary

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Dit protocol beschrijft experimentele procedures karakteriseren van genoom-brede veranderingen in de niveaus van Histon posttranslationele modificaties (PTM) die zich voordoen in verband met de overexpressie van eiwitten ALS en Parkinson in zijn gekoppeld Saccharomyces cerevisiae modellen. Na SDS-pagina de scheiding, worden individuele Histon PTM niveaus gedetecteerd met wijziging-specifieke antilichamen via het westelijke bevlekken.

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Cite this Article

Bennett, S. A., Cobos, S. N., Meykler, M., Fallah, M., Rana, N., Chen, K., Torrente, M. P. Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models. J. Vis. Exp. (145), e59104, doi:10.3791/59104 (2019).

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