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JoVE Journal Genetics
墨西哥鱼基因功能的操纵
 
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墨西哥鱼基因功能的操纵

Article DOI: 10.3791/59093-v • 07:01 min • April 22nd, 2019
April 22nd, 2019 •
1,2, 1,2, 1,2, 2,3, 1,2, 2,3
1Department of Biological Sciences, Florida Atlantic University, 2Jupiter Life Science Initiative, Florida Atlantic University, 3Harriet L. Wilkes Honors College, Florida Atlantic University

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我们描述了在进化模型系统中操纵基因的方法. 描述了三种不同的技术: Tol2 介导的转基因、使用 CRISPR/Cas9 对基因组进行有针对性的操纵以及使用吗啡酮敲除表达。这些工具应有助于直接研究表面和洞穴居住形式之间变异背后的基因。

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遗传学 第146期 生命科学 生命科学 (一般) 犹太 CRISPR 基因编辑 转基因 吗啡 击倒
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