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Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

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11:20 min

March 02, 2019

DOI:

11:20 min
March 02, 2019

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Summary

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Here we report common methods to analyze the phagocytic function of murine alveolar macrophages and bacterial clearance from the lung. These methods study in vitro phagocytosis of fluorescein isothiocyanate beads and in vivo phagocytosis of Pseudomonas aeruginosa Green Fluorescent Protein. We also describe a method for clearing P. aeruginosa in mice.

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