/

/

硫醇反应试剂的合成与生物共轭作用--用于原位选择性修饰免疫共轭的生成

JoVE Journal
Química

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Química

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Please note that all translations are automatically generated. Click here for the English version.

硫醇反应试剂的合成与生物共轭作用--用于原位选择性修饰免疫共轭的生成

9,076 Views

•

08:47 min

•

March 06, 2019

DOI:

10.3791/59063-v

DOI:

10.3791/59063-v

•

08:47 min

March 06, 2019

•

2 Views

Maria Davydova¹, Guillaume Dewaele Le Roi^1,2, Pierre Adumeau¹, Brian M. Zeglis^1,2,3,4

¹Department of Chemistry,Hunter College of the City University of New York, ²Ph.D. Program in Chemistry,Graduate Center of the City University of New York, ³Department of Radiology,Memorial Sloan Kettering Cancer Center, ⁴Department of Radiology,Weill Cornell Medical College

Summary

在本议定书中, 我们将描述 pods 的合成, 这是一种基于苯氧二唑基甲基磺酸试剂的合成, 用于将货物选择性地附着在生物分子的硫醇中, 特别是抗体中。此外, 我们将描述含有 pod 的双官能螯合剂的合成和表征及其与模型抗体的结合。

Explore More Videos

Thiol-reactive Reagents Site-specific Bioconjugation Immunoconjugates PODS Maleimide-thiol-based Bioconjugation Aminophenyl Oxadiazole Thiol Diisopropylethylamine Iodomethane Boc-protected Carboxylic Acid Dichloromethane EDCI Aniline Hydrochloric Acid Sodium Carbonate Magnesium Sulfate Ethyl Acetate Cyclohexane Carbamate M-chloroperbenzoic Acid

Read Article