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心脏细胞治疗中干细胞 dna 完整性的评估
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JoVE Journal Genética
Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy
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心脏细胞治疗中干细胞 dna 完整性的评估

DOI:
10.3791/58971-v

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10:16 min

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January 25, 2019

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, , , ,
1Department of Biomedical Engineering, School of Engineering, School of Medicine,University of Alabama at Birmingham

Capítulos

  • 00:04Título
  • 00:50Comet Assay
  • 03:19DNA Damage Response
  • 06:12Counting of the DNA Damage Response (DDR) Foci
  • 08:11Results: DNA Damage Response in Human iPS Cells and iPS-derived Cardiomyocytes
  • 09:43Conclusion

Summary

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在这里, 我们提供了一个实验设置的详细描述, 用于分析细胞移植前干细胞 dna 完整性的评估。

Tags

DNA IntegrityStem Cell Quality AssessmentComet AssayDNA DamageRegenerative TherapyCardiomyopathyInduced Pluripotent Stem CellsReactive Oxygen SpeciesCell TransplantationDNA-RNA Free WaterAgaroseComet SlideLysis BufferAlkaline SolutionElectrophoresisEthanol

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