Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Jessica M. Miller; Nikhil Maneesh Mardhekar; Vasanthi Rajasekaran; Jianyi Zhang; Ram Kannappan

doi:10.3791/58971

Journal

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Genetik

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Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

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Genetik

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JoVE Journal Genetik

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

7,508 Views

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10:16 min

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January 25, 2019

DOI:

10.3791/58971-v

DOI:

10.3791/58971-v

•

10:16 min

January 25, 2019

•

2 Views

Jessica M. Miller*¹, Nikhil Maneesh Mardhekar*¹, Vasanthi Rajasekaran¹, Jianyi Zhang¹, Ram Kannappan¹

¹Department of Biomedical Engineering, School of Engineering, School of Medicine,University of Alabama at Birmingham

Summary

Here, we provide a detailed description of an experimental setup for an analysis of the assessment of DNA integrity in stem cells prior to cell transplantation.

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DNA Integrity Stem Cell Quality Assessment Comet Assay DNA Damage Regenerative Therapy Cardiomyopathy Induced Pluripotent Stem Cells Reactive Oxygen Species Cell Transplantation DNA-RNA Free Water Agarose Comet Slide Lysis Buffer Alkaline Solution Electrophoresis Ethanol

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Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

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