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Biologia do Desenvolvimento
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Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

DOI:
10.3791/58965-v

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14:23 min

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February 16, 2019

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,
1Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina,Universidade de Lisboa

Capítulos

  • 00:00Título
  • 02:22Isolation of Quail Endoderm Containing the Prospective Domain of the Thymic Rudiment
  • 07:41Isolation of Chicken Somatopleura Mesoderm
  • 10:36In Vitro Organotypic Assay: Heterospecific Association of Quail ¾PP Endoderm and Chicken Somatopleura Mesoderm
  • 12:13Representative Results
  • 13:49Conclusion

Summary

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This article provides a method to isolate pure embryonic tissues from quail and chicken embryos that can be combined to form ex vivo chimeric organs.

Tags

Quail-chicken Chimeric OrgansThymus FormationIn Vitro Organotypic AssayTranscriptome AnalysisDevelopmental ProcessesPharyngeal PouchesSomatopleura Mesoderm

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