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ステンレス高品質レーザーからの RNA の隔離のプロトコルをキャプチャ人間死後小脳 Microdissected プルキンエ細胞
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09:04 min
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January 17, 2019
DOI:
1Department of Pathology and Cell Biology,Columbia University, 2Division of Movement Disorders, Department of Neurology,Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health,Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine,Yale University
Summary
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このプロトコルは、視覚化し、キャプチャ レーザーマイクロダイ死後人間小脳から新鮮凍結組織におけるプルキンエ細胞を分離する染色無料のアプローチを使用します。このプロトコルの目的は、RNA 配列のため質の高い RNA の十分な量を生成することです。
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Cite this Article
Martuscello, R. T., Louis, E. D., Faust, P. L. A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum. J. Vis. Exp. (143), e58953, doi:10.3791/58953 (2019).
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